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Pig Intestinal Membrane‐Bound Receptor (Guanylyl Cyclase) for Heat‐Stable Enterotoxin: cDNA Cloning, Functional Expression, and Characterization
Author(s) -
Wada Akihiro,
Hirayama Toshiya,
Kitao Saori,
Fujisawa Junichi,
Hidaka Yuji,
Shimonishi Yasutsugu
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01819.x
Subject(s) - biology , complementary dna , cloning (programming) , enterotoxin , heat stable enterotoxin , molecular cloning , guanylate cyclase 2c , receptor , microbiology and biotechnology , gene , biochemistry , guanylate cyclase , escherichia coli , computer science , programming language
A cDNA encoding the receptor protein for a heat‐stable enterotoxin (STa) produced by enterotoxigenic Escherichia coli was cloned from intestinal epithelial cells of a 10‐week‐old pig. The cDNA had an open reading frame of 3,219 base pairs and coded for a protein with 1,073 amino acid residues. The mature protein consisted of 1,050 amino acid residues with a molecular mass of ca. 121 kDa and was 87% and 82% identical with the human and rat protein, respectively. The CHO cell line overexpressing the pig recombinant STa receptor specifically bound to a photoaffinity‐labeled analog of STa and showed marked elevation of the cellular content of cGMP in response to STa.