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Existence of a Novel Hemagglutinin Having No Protease Activity in Vibrio mimicus
Author(s) -
Alam Munirul,
Miyoshi Shinichi,
Maruo Ikuyo,
Ogawa Chiemi,
Shinoda Sumio
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01809.x
Subject(s) - protease , biology , hemagglutinin (influenza) , microbiology and biotechnology , enzyme , vibrio , proteolytic enzymes , vibrionaceae , biochemistry , virology , bacteria , virus , genetics , gene
The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well‐known HA/protease. A non‐protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA ( V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.