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Specific Detection of a Verotoxin 2 Variant, VT2vp1, by a Bead‐Enzyme‐Linked Immunosorbent Assay
Author(s) -
Cao Chunyu,
Yamasaki Shinji,
Lin Zaw,
Kurazono Hisao,
Takeda Yoshifumi
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01804.x
Subject(s) - verocytotoxin , escherichia coli , biology , enzyme , bead , microbiology and biotechnology , enterobacteriaceae , polymerase chain reaction , toxin , biochemistry , gene , materials science , composite material
Abstract A bead‐enzyme‐linked immunosorbent assay to specifically detect a Verotoxin 2 variant, VT2vpl, was developed. The sensitivity of the bead‐ELISA was 200 pg/ml of the purified VT2vpl and it did not react with 20 ng/ml of the purified VT2. The specificity of the bead‐ELISA was examined with 107 strains of Verocytotoxin‐producing Escherichia coli that include VT1‐, VT2‐, VT2vha‐, VT2vhb‐ and VT2vp1‐producing E. coli , and only VT2vp1‐producing E. coli that were confirmed by VT2vp1‐specific polymerase chain reaction gave positive results. It was noted that all 58 VT2vp1‐producing E. coli strains were from pigs, but not from cows and humans.