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Inhibition of Candida albicans Growth by Murine Peritoneal Neutrophils and Augmentation of the Inhibitory Activity by Bacterial Lipopolysaccharide and Cytokines
Author(s) -
Tansho Shigeru,
Abe Shigeru,
Yamaguchi Hideyo
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01794.x
Subject(s) - candida albicans , biology , lipopolysaccharide , microbiology and biotechnology , tumor necrosis factor alpha , cytokine , granulocyte , macrophage , interferon gamma , in vitro , colony stimulating factor , neutrophile , granulocyte macrophage colony stimulating factor , immunology , inflammation , haematopoiesis , biochemistry , stem cell
Abstract Anti‐ Candida activity of murine neutrophils and its regulation by immunomodulators were studied in vitro. Murine neutrophils which were prepared from peritoneal‐exudated cells inhibited the growth of Candida albicans at an effector: target (E/T) ratio of 30/1 or above. This anti‐ Candida activity of neutrophils was augmented by lipopolysaccharide from Escherichia coli , murine tumor necrosis factor (TNF), murine interferon‐γ (IFN‐γ) and murine granulocyte macrophage colony‐stimulating factor (GM‐CSF) but not by granulocyte colony‐stimulating factor (G‐CSF) added to the incubation medium. Greater extent of augmentation was obtained when TNF plus GM‐CSF or INF‐γ plus GM‐CSF were used in combination. These results indicate that anti‐ Candida activity of murine neutrophils is regulated similarly to that of the human neutrophils reported previously. Therefore murine peritoneal neutrophils can be used as a favorable substitute for human neutrophils in studies on protective machinery against C. albicans infection.