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Transfer of Protection to Murine Typhoid Conferred by L‐Form Salmonella typhimurium in Dependence of Cooperation between L Form‐Adopted Macrophages and L Form‐Induced Lyt‐2 + T Cells
Author(s) -
Nishikawa Fumiko,
Kita Eiji,
Matsui Norio,
Kashiba Shuzo
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01765.x
Subject(s) - biology , salmonella , typhoid fever , microbiology and biotechnology , salmonella typhi , enterobacteriaceae , virology , bacteria , escherichia coli , genetics , gene
The effector cells responsible for protection to Salmonella typhimurium in C3H/HeJ mice, conferred by L‐form S. typhimurium , were determined by cell transfer test. Nonfractionated spleen cells from 6‐week immune mice but not from 24‐week immune animals transferred anti‐ S. typhimurium immunity. Treatment with anti‐macrophage antiserum and complement most effectively abolished protective capacity in 6‐week immune cells, while anti‐T cell monoclonal antibody plus complement reduced it to a lesser extent. However, adoptive protection was achieved only by transfer of immune macrophages along with Lyt‐2 + T cells selected from 6‐week immune spleen cells. These Lyt‐2 + T cells were cytotoxic to Kupffer cells from C3H/HeJ mice which had been infected 48 hr previously and from the mice which had been immunized 1 week previously, but not to the cells from 6‐week immune mice and from normal animals. Moreover, protective capacity in immune macrophages seemed to be correlated to the degree of colonization by the L forms, and the inability to transfer immunity of 24‐week immune spleen cells may be due to the decrease in the L form‐colonization. These results suggest that cooperation between the L form‐colonized macrophages and L form‐induced cytotoxic Lyt‐2 + T cells contributes to anti‐ S. typhimurium immunity, and might imply the immunological difference between the 6‐week immune phagocytes and the cells at an early stage of infection or immunization.