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Identification of Hepatitis C Virus Subtype 3b (HCV‐3b) among Japanese Patients with Liver Diseases Using Highly Efficient Primers for Reverse Transcription‐Polymerase Chain Reaction
Author(s) -
Doi Hisaya,
Yoon Seitetsu,
Homma Morio,
Hotta Hak
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01759.x
Subject(s) - biology , polymerase chain reaction , reverse transcriptase , virology , reverse transcription polymerase chain reaction , hepatitis c virus , virus , polymerase , nucleic acid sequence , hepatitis b virus dna polymerase , flaviviridae , genome , microbiology and biotechnology , genetics , dna , gene , messenger rna
With a reverse transcription‐polymerase chain reaction method that utilized highly efficient primers, the NS5 region of hepatitis C virus (HCV) genome could be amplified from all of the 24 serum samples randomly obtained from Japanese patients with liver diseases. Subtype analysis on the basis of nucleotide sequence similarities of the amplified fragments revealed that 2 out of 24 Japanese patients (8.3%) were infected with the recently identified subtype HCV‐3b, which had been thought to be rare in Japan.