Premium
Diversity in Molecular Mass of the Common EDTA‐Soluble Antigens of Clostridium chauvoei and Clostridium septicum
Author(s) -
Hamaoka Takafumi,
Terakado Nobuyuki,
Nakamura Shinichi
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01756.x
Subject(s) - clostridium septicum , biology , antigen , monoclonal antibody , epitope , molecular mass , microbiology and biotechnology , immunofluorescence , antibody , clostridium , biochemistry , bacteria , immunology , enzyme , genetics
Common EDTA‐soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect‐immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of C. septicum , in IFA. In immunoblot analysis with the mAbs, the bands at molecular mass of 35 kDa were found in all C. chauvoei strains, while the bands at 36 kDa were found in 4 of 5 strains of C. septicum. These results indicate that the 35 kDa antigen of C. chauvoei and the 36 kDa antigen of C. septicum possess a similar epitope recognized by the mAb.