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Mechanism of Inhibition of DNA Synthesis by 1‐β‐ D ‐Arabinofuranosyl‐ E ‐5‐(2‐Bromovinyl)uracil
Author(s) -
Suzutani Tatsuo,
Machida Haruhiko,
Honess Robert W.
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03244.x
Subject(s) - klenow fragment , uracil , biology , dna polymerase i , terminator (solar) , dna , escherichia coli , microbiology and biotechnology , dna synthesis , dna polymerase , biochemistry , mechanism of action , stereochemistry , chemistry , polymerase chain reaction , in vitro , gene , exonuclease , reverse transcriptase , ionosphere , physics , astronomy
The mechanism of the inhibitory action of 1‐β‐ D ‐arabinofuranosyl‐ E ‐5‐(2‐bromovinyl) uracil triphosphate (BV‐araUTP) on DNA synthesis by Escherichia coli DNA polymerase I Klenow fragment was studied. Acting as a chain terminator, BV‐araUTP inhibited DNA synthesis by Klenow fragment more effectively than 2′, 3′‐dideoxythymidine triphosphate (ddTTP). However, the incorporation sites of BV‐araU monophosphate were restricted at consecutive dTMP sequence whereas ddTMP was incorporated at every dTMP site.