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A Positive Correlation between the Precursor Frequency of Cytotoxic Lymphocytes to Autologous Epstein‐Barr Virus‐Transformed B Cells and Antibody Titer Level against Epstein‐Barr Virus‐Associated Nuclear Antigen in Healthy Seropositive Individuals
Author(s) -
Kusunoki Yoichiro,
Huang Hua,
Fukuda Yasuko,
Ozaki Kyoko,
Saito Mayumi,
Hirai Yuko,
Akiyama Mitoshi
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03237.x
Subject(s) - cytotoxic t cell , peripheral blood mononuclear cell , epstein–barr virus , titer , virus , antigen , biology , antibody , virology , cytotoxicity , immunology , in vitro , biochemistry
A limiting dilution analysis was established to determine the precursor frequency (PF) of cytotoxic lymphocytes against autologous B cells transformed with the Epstein‐Barr virus (EBV). This method was found to detect mainly self‐restricted T‐cell activity and little non‐self‐restricted cytotoxicity. The mean PF in 21 healthy EBV‐seropositive persons was 1.4 × 10‐ 3 (range: 0.03 × 10‐ 3 to 8.7 × 10‐ 3 ) for peripheral blood mononuclear cells, whereas 4 samples of mononuclear cells obtained from umbilical cord blood had PFs below 0.007 × 10‐ 3 . A positive correlation was observed between the PF and serum antibody titers against EBV‐associated nuclear antigen among the seropositive persons.