Premium
Cloning and Sequencing of Two New Verotoxin 2 Variant Genes of Escherichia coli Isolated from Cases of Human and Bovine Diarrhea
Author(s) -
Lin Zaw,
Yamasaki Shinji,
Kurazono Hisao,
Ohmura Mari,
Karasawa Tadahiro,
Inoue Takahiro,
Sakamoto Seisuke,
Suganami Takahiro,
Takeoka Tomohiro,
Taniguchi Yoshihiro,
Takeda Yoshifumi
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03236.x
Subject(s) - verocytotoxin , biology , escherichia coli , gene , cloning (programming) , genetics , strain (injury) , homologous chromosome , nucleic acid sequence , microbiology and biotechnology , enterobacteriaceae , anatomy , computer science , programming language
Abstract We cloned and sequenced two new Verotoxin 2 (VT2) variant genes: one from an Escherichia coli strain from a case of bovine diarrhea and the other from an E. coli strain from a patient with diarrhea. The nucleotide and amino acid sequences of these two genes were highly homologous with, but distinct from those of the VT2, VT2vha, VT2vhb, SLT‐IIv (VT2vp1) and SLT‐IIva (VT2vp2) genes. Their nucleotide sequences were much more closely homologous to that of VT2vh than to that of VT2vp. Search for these two new genes in other Verocytotoxin‐producing E. coli strains resulted in the isolation of 2 strains carrying one of the new VT2 variant genes, one strain from Tokyo and the other from Canada.