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Differentiation of Rickettsia japonica by Restriction Endonuclease Fragment Length Polymorphism Using Products of Polymerase Chain Reaction Amplification with Rickettsia rickettsii 190‐Kilodalton Surface Antigen Gene Primers
Author(s) -
Yan Yansheng,
Uchiyama Tsuneo,
Uchida Takahiro
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03234.x
Subject(s) - biology , polymerase chain reaction , rickettsia rickettsii , restriction fragment length polymorphism , restriction enzyme , microbiology and biotechnology , gene , endonuclease , rickettsiaceae , rickettsia , cleaved amplified polymorphic sequence , virology , rickettsiales , genetics , spotted fever , virus
Restriction fragment length polymorphism of polymerase chain reaction (PCR) amplification products differentiated Rickettsia japonica , a causative agent of Oriental spotted fever, from other spotted fever group (SFG) rickettsiae. Primer pair Rr 190. 70p and Rr 190. 602n of R. rickettsii 190‐kDa antigen gene sequence primed genomic DNAs obtained from R. japonica , type strain YH and strains NT, NK, YKI, and TKN. The products were cleaved by Pst I but not by Afa I restriction endonuclease. The Pst I digestion pattern of PCR‐products amplified from all strains of R. japonica was identical and easily differentiated from that of other SFG rickettsiae. The present study demonstrated a genotypic difference between R. japonica and other pathogenic SFG rickettsiae.