Premium
Studies on Hemolytic Action of a Hemolysin Produced by Vibrio mimicus
Author(s) -
Shinoda Sumio,
Ishida Kazuhiko,
Oh EunGyoung,
Sasahara Kazuhiro,
Miyoshi ShinIchi,
Chowdhury Mohammad Afzalur Rahim,
Yasuda Tatsuji
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03229.x
Subject(s) - biology , hemolysin , microbiology and biotechnology , vibrio , hemolysis , vibrionaceae , action (physics) , genetics , bacteria , virulence , immunology , gene , physics , quantum mechanics
Some properties and mechanism of action of a hemolysin (VMH) produced by an enteropathogenic Vibrio mimicus strain was examined. VMH was heat‐labile and inhibited by addition of divalent cations, including Ca 2+ , Mg 2+ and Mn 2+ . The hemolysis by VMH was inhibited by incubating with gangliosides, suggesting that the ganglioside was the binding site on the erythrocyte membrane for VMH. Existence of a galactose moiety on reducing end of the ganglioside molecule and a sialic acid on the galactose moiety was suggested to be important for the binding of VMH molecule. Colloid osmotic manner of the hemolysis by VMH was demonstrated.