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Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike
Author(s) -
Fujii Nobuhiro,
Kimura Kouichi,
Yokosawa Noriko,
Oguma Keiji,
Yashiki Teruo,
Takeshi Kouichi,
Ohyama Touru,
Isogai Emiko,
Isogai Hiroshi
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03227.x
Subject(s) - clostridium butyricum , nucleic acid sequence , biology , strain (injury) , clostridium botulinum , open reading frame , peptide sequence , gene , amino acid , nucleotide , plasmid , clostridium , microbiology and biotechnology , toxin , genetics , biochemistry , bacteria , anatomy
The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb Hin dIII fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C.butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.