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Detection of Dengue Virus Antigens in Cultured Cells by Using Protein A‐Gold‐Silver Staining (pAgs) Method
Author(s) -
Chen WeiJune,
Chen SuLih,
Fang AyHuey,
Wang MingTsan
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03222.x
Subject(s) - dengue virus , virology , dengue fever , biology , virus , antigen , clone (java method) , staining , antibody , inoculation , immunology , dna , genetics
A protein A‐gold‐silver (pAgs) staining was developed to detect dengue virus antigens in cultured cells. The method can be carried out in either newly‐subcultured or monolayered cells. Dengue virus‐inoculated C6/36 clone of Aedes albopictus cells and human endothelial cells appeared brown‐yellowish color on the peripheral membrane of the infected cells. In many cases, the infected C6/36 cells appeared darker than that of the infected endothelial cells. The positive results from the inoculated C6/36 cells usually appeared as early as 2 days post‐inoculation for types 1, 2, and 4 of dengue viruses and 3 days for the dengue 3 virus. The same batch of specimens detected by direct immunofluorescence antibody test (DFA) showed positive 4 days post‐inoculation for the types 2, 3, and 4 of dengue viruses and 6 days for the dengue 1 virus. The result also showed that all pAgs‐positive specimens were also DFA‐positive, but not vice versa. It suggested that pAgs is not only sensitive but also specific for dengue virus detection from inoculated cultured cells.
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