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In Vitro Modification of Human Immunodeficiency Virus Type 1 (HIV‐1) Infectivity by the U937 Cells
Author(s) -
ElFarrash Mohamed A.,
Masuda Takao,
Kuroda Marcelo J.,
Harada Shinji
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03221.x
Subject(s) - infectivity , biology , u937 cell , virology , cell culture , virus , clone (java method) , glycoprotein , viral entry , in vitro , viral replication , microbiology and biotechnology , genetics , gene
The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV‐1) was studied by infecting a monoblastoid cell line (U937) or a T‐cell line (MOLT‐4) with a highly infective single clone of HIV‐1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infected U937 cells consistently produced viruses with minimal infectivity. This phenotypic change was host‐dependent as the back‐passage of the U937‐produced low infective viruses into MOLT‐4 cells resulted in regaining their original high infectivity. Southern and Northern blot analyses of the HIV‐1 grown in U937 cells did not reveal any genomic difference between it and the virus grown it MOLT‐4 cells. The radioimmunoprecipitation analysis of viral proteins showed that the HIV‐1‐infected U937 cells had a different pattern of envelope glycoproteins and core proteins, which well correlated with the low infectivity of the produced viruses. This experimental system using MOLT‐4 and U937 cell lines would be useful to further explore host cell factor(s) which play an important role in the regulation of HIV‐1 infectivity.