Premium
Chemical Analysis of Lipopolysaccharides of Shigella sonnei Form II Strains Expressed by Cloned Form I Antigen Genes
Author(s) -
Okamura Noboru,
Chida Toshio,
Kinoshita Masao,
Yoshida Yoko,
Kondo Seiichi,
Hisatsune Kazuhito
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03218.x
Subject(s) - heptose , shigella sonnei , biology , antigen , lipopolysaccharide , microbiology and biotechnology , sugar acids , plasmid , shigella , gene , galactose , enterobacteriaceae , biochemistry , escherichia coli , sugar , genetics , immunology , mutant
A compositional sugar analysis was carried out on lipopolysaccharide (LPS) from Shigella sonnei form II in which a plasmid with cloned form I antigen genes had been introduced. The recipient form II strains contained galactose, glucose, heptose, glucosamine, and 2‐keto‐3‐deoxyoctonic acid (KDO) (2: 3: 1: 2: 2) in its LPS, while the transformant form I LPS contained, besides these sugars, N ‐acetyl‐ L ‐altrosaminouronic acid as an additional sugar constituent, which is known to be one of the antigenic determinants of form I antigen.