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Cytotoxic Effect of Hemolytic Culture Supernatant from Enterococcus faecalis on Mouse Polymorphonuclear Neutrophils and Macrophages
Author(s) -
Miyazaki Shuichi,
Ohno Akira,
Kobayashi Intetsu,
Uji Tatsuya,
Yamaguchi Keizo,
Goto Sachiko
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03209.x
Subject(s) - enterococcus faecalis , hemolysin , lysis , biology , microbiology and biotechnology , hemolysis , phosphatidylcholine , sphingomyelin , cytolysis , bacteria , staphylococcus aureus , biochemistry , cytotoxicity , immunology , in vitro , membrane , phospholipid , virulence , gene , genetics
We reconfirmed that the LD 50 S of hemolytic Enterococcus faecalis strains were significantly less than those of nonhemolytic E. faecalis strains in normal mice. Hemolysin produced by E. faecalis lysed human, horse, rabbit, and mouse erythrocytes, but not cow and sheep erythrocytes. Sphingomyelin comprises a part of the lipid composition of the erythrocyte membrane of all mammalian species tested. But phosphatidylcholine exists only in human, horse, rabbit, and mouse. These two lipids inhibited lysis of horse erythrocytes by hemolytic E. faecalis . Phosphatidylcholine is probably the binding component on the membrane of erythrocytes for E. faecalis hemolysin. The hemolytic culture supernatant lysed not only erythrocytes but also mouse polymorphonuclear neutrophils (PMNs) and macrophages.