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Localization and Functions of Japanese Encephalitis Virus Nonstructural Proteins NS3 and NS5 for Viral RNA Synthesis in the Infected Cells
Author(s) -
Edward Zulkarnain,
Takegami Tsutomu
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03206.x
Subject(s) - ns3 , flavivirus , biology , virology , japanese encephalitis , rna , virus , in vitro , immunofluorescence , western blot , viral replication , antiserum , flaviviridae , microbiology and biotechnology , encephalitis , antibody , biochemistry , gene , hepatitis c virus , immunology
Recently it has been reported that Japanese encephalitis virus (JEV)‐specific RNAs can be synthesized in vitro in the subcellular fraction including outer‐nuclear membrane (Takegami and Hotta, 1989). The results of Western blot analysis and indirect immunofluorescence test using two kinds of monospecific antisera against JEV nonstructural proteins NS3 and NS5 showed that NS3 and NS5 were membrane‐associated proteins and formed the complex at the perinuclear site in the infected cells. Both antisera against NS3 and NS5 inhibited in vitro RNA synthesis. These results suggest that NS5 and NS3 play important role(s) in flavivirus RNA replication.