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Role of Calcium in Biphasic Germination of Bacillus cereus T Spores Sensitized to Lysozyme
Author(s) -
Shibata Hirofumi,
Adachi Sawako,
Hirose Yoko,
Ike Mika,
Tani Isamu,
Hashimoto Tadayo
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03199.x
Subject(s) - dipicolinic acid , dithiothreitol , divalent , bacillus cereus , spore , germination , lysozyme , nuclear chemistry , phase (matter) , calcium , bacillales , chelation , biology , chemistry , biochemistry , microbiology and biotechnology , inorganic chemistry , bacteria , botany , enzyme , genetics , organic chemistry , bacillus subtilis
Biphasic germination induced by inosine in the presence of Ca 2+ was examined using Bacillus cereus T spores treated with sodium dodecyl sulfate (SDS) and dithiothreitol (DTT) at pH 10. The first phase of the germination was stimulated by Ca 2+ in the concentration‐dependent manner, showing the optimal concentration at 0.5‐1.0 mM. The second phase appeared to be insensitive to the cation. The optimal temperatures for the first and the second phase were 25 C and 40 C, respectively; the optimal pHs for the two phases were 7‐9 and around 7.5, respectively. Heat resistance and dipicolinic acid of the SDS‐DTT‐treated spores were lost mostly during the first phase. A Ca 2+ ‐specific chelator, glycoletherdiamine‐ N,N,N',N' ‐tetraacetic acid (GEDTA), inhibited the first phase evoked by Ca 2+ , while it had no inhibitory effect on the second phase. In contrast, the divalent cations examined, except Mg 2+ and Sr 2+ , affected not only the first phase but also the second phase. The order of inhibitory effect on the first phase was Hg 2+ > Zn 2+ > Ba 2+ , Co 2+ , Cu 2+ > Mn 2+ ; on the second phase, it was Hg 2+ > Cu 2+ > Zn 2+ > Co 2+ > Mn 2+ > Ba 2+ .