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Cytotoxicity Induced by Monoclonal Antibody to α‐Fetoprotein Coadministered with Spleen Cells of Nude Mice
Author(s) -
Masuda Kazuyoshi,
Nagata Shunji,
Hirano Koichiro,
Takagishi Yasushi,
Hirai Hidematsu
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03195.x
Subject(s) - antibody dependent cell mediated cytotoxicity , spleen , monoclonal antibody , biology , cytotoxicity , nude mouse , effector , cell culture , antibody , immunology , medicine , microbiology and biotechnology , cancer research , in vitro , biochemistry , genetics
This paper describes an attempt to effectively induce antibody‐dependent cell‐mediated cytotoxicity (ADCC) in nude mice. A monoclonal antibody against α‐fetoprotein, 80G, coadministered with spleen cells from other nude mice bearing HuH‐7N (xenograft of human hepatoma cell line, HuH‐7) significantly suppressed the growth of HuH‐7N as compared to treatment with 80G alone. 80G with spleen cells from normal nude mice also had some suppressive effect. In contrast, no effect was observed with each spleen cells alone as well as 80G alone. These results suggest that further supply of effector cells could enhance ADCC activity in nude mice.