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Detection of Verocytotoxin from Stool and Serological Testing of Patients with Diarrhea Caused by Escherichia coli O157: H 7
Author(s) -
Yamada Sumio,
Matsushita Shigeru,
Kai Akemi,
Sasaki Michiko,
Tsuji Atsutoshi,
Kanemitsu Takefumi,
Yamashita Naoya,
Anzai Eiko,
Kudoh Yasuo
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03187.x
Subject(s) - verocytotoxin , vtec , serology , flagellin , feces , microbiology and biotechnology , serotype , antibody , outbreak , antigen , biology , escherichia coli , diarrhea , virology , counterimmunoelectrophoresis , medicine , immunology , bacteria , biochemistry , genetics , gene
The detecion of verocytotoxin (VT) in stool and measurement of antibodies against VT and three antigens (unheated‐antigen, LPS, and flagellin) of Escherichia coli O157: H7 in the serum of patients with diarrhea were examined. Five of 14 inpatients during an outbreak had fecal VT2 in stool taken within 5 days of onset to hospitalization. Among these 5, 3 of them also had fecal VT‐producing E. coli (VTEC) serotype O157: H7, whereas the other 2 did not. In the passive hemagglutination (PHA) test with formalinized sheep red blood cells sensitized with theee VTEC O157: H7 antigens, 49 (74.2%) of 66 outbreak patients and 3 of 3 sporadic cases had antibodies against both or one of unheated‐antigen and LPS of E. coli O157, but none had antibody against flagellin. In addition, anti‐VT2 antibody was demonstrated in serum samples from 15 (94%) of 16 inpatients and 2 (4%) of 50 outpatients in an outbreak by a VT‐enzyme‐linked immunosorbent assay (VT‐ELISA). These results showed that serological assay particularly for antibodies against VT and unheated‐antigen or LPS of VTEC O157 may provide a useful tool for diagnosis of infection with VTEC O157.