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Genetic Transformation in Helicobacter pylori
Author(s) -
Tsuda Masataka,
Karita Mikio,
Nakazawa Teruo
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03184.x
Subject(s) - plasmid , electroporation , biology , transformation (genetics) , helicobacter pylori , escherichia coli , recombinant dna , dna , microbiology and biotechnology , homologous recombination , genetics , plasmid preparation , gene , pbr322
Genetic transformation in Helicobacter pylori was investigated by using its chromosomal and plasmid DNAs. Six out of the eight strains exhibited the natural competence for incorporation of H. pylori chromosomal DNA, and all the strains incorporated the donor DNA efficiently by washing and concentrating the cells, with a glycerol solution. The much higher frequency of transformation was obtained in each strain by means of electroporation. Electroporation experiments were also conducted by use of the recombinant DNAs consisting of the H. pylori and Escherichia coli plasmids as the donors, and the occurrence of the homologous recombination was demonstrated between the incoming H. pylori plasmid‐derived region and the corresponding region of the originally residing plasmid in H. pylori .