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Construction of a Slime Negative Transposon Mutant in Staphylococcus epidermidis Using the Enterococcus faecalis Transposon Tn 917
Author(s) -
Grüter Ludwig,
Feucht Hubert,
Mempel Martin,
Laufs Rainer
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb03176.x
Subject(s) - transposon mutagenesis , biology , transposable element , staphylococcus epidermidis , mutant , microbiology and biotechnology , enterococcus faecalis , transformation (genetics) , mutagenesis , genetics , bacteria , staphylococcus aureus , gene
Abstract The slime‐producing Staphylococcus epidermidis strain sensu strictu CNS23 was transformed by protoplast transformation with the plasmid pTV1 which carries transposon Tn 917 . Using this transposon mutagenesis system we obtained the Tn 917 ‐inserted mutant CT512, which has lost the ability to produce slime. A single insertion of the trasposon Tn 917 into the chromosome of CT512 could be detected by Southern hybridization. This mutant showed a significantly higher stability concerning its slime‐negative phenotype compared with spontaneous slime‐negative mutants of S. epidermidis strain CNS23. In slime‐ELISA no slime‐associated antigen could be detected in extracts of the transposon mutant. Compared to slime‐positive S. epidermidis strains, CT512 lacked in accumulative growth in microtiter tube test.