Premium
Influence of a Small Number of Mature T Cells in Donor Bone Marrow Inocula on Reconstitution of Lymphoid Tissues and Negative Selection of a T Cell Repertoire in the Recipient
Author(s) -
AraseFukushi Noriko,
Arase Hisashi,
Wang Bingyan,
Hirano Mari,
Ogasawara Kazumasa,
Good Robert A.,
Onoé Kazunori
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01720.x
Subject(s) - biology , chimera (genetics) , bone marrow , clonal deletion , microbiology and biotechnology , antigen , monoclonal antibody , immunology , negative selection , congenic , t cell , antibody , immune system , t cell receptor , genetics , gene , genome
Allo‐chimerism and clonal elimination of self antigen (Ag) (Ia + Mls‐1 a ) reactive Vβ6+ T cells were analyzed and compared between allogeneic bone marrow (BM) chimeras reconstituted with BM cells which had been treated with anti‐Thy‐1 monoclonal antibody (mAb) plus complement (C) (T – chimeras) and BM chimeras which had been reconstituted with BM cells pretreated with anti‐Thy‐1 mAb alone (T + chimeras). When lethally irradiated AKR (Mls‐1 a ) mice were reconstituted with BM cells from B10 or B10 H‐2 congenic mice, both T + and T – chimeras were entirely free of signs of graft‐versus‐host reaction (GVHR). However, complete replacement of the AKR lymphoid tissues by donor BM cells was accomplished at an early stage in T + chimeras but not in T – chimeras. On the other hand, clonal elimination of Vβ6 + T cells reactive to the recipient Ag (Mls‐1 a ) was abolished in T + chimeras but successfully induced in T – chimeras. The Vβ6 + T cells not eliminated in T + chimeras showed depressed responses against Mls‐1 a antigens. The findings herein demonstrate that T cells which contaminate a BM inoculum survive in recipient mice after treatment with anti‐Thy‐1 mAb without C in vitro followed by BMT. The surviving T cells have been estimated to represent fewer than 0.5% of the BM cells inoculated. These cells appear to accelerate the full replacement of recipient lymphoid tissues by donor cells. Furthermore, the T cells which survive in the marrow inoculum influence eventually the development of a tolerant state in the T cell repertoire of the donor.