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Granulocyte‐Macrophage Colony‐Stimulating Factor and Tumor Necrosis Factor Alpha in Patients with Human Immunodeficiency Virus (HIV) Type 1 Infection
Author(s) -
Hober Didier,
Ajana Faïza,
Petit MarieCécile,
Sartiaux Claudine,
Boniface Michel,
Caillaux Michèle,
Mouton Yves,
Wattre Pierre,
ManiezMontreuil Michèle
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01706.x
Subject(s) - tumor necrosis factor alpha , immunology , cytokine , granulocyte macrophage colony stimulating factor , asymptomatic , peripheral blood mononuclear cell , medicine , stage (stratigraphy) , biology , lymphocyte , gastroenterology , in vitro , paleontology , biochemistry
Variations in cytokine production in patients with human immunodeficiency virus (HIV) infection could be involved in the physiopathology and in the progression of the disease. Therefore we studied the level of granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) and tumor necrosis factor α (TNF α ) produced in patients with HIV infection at stage II (asymptomatic seropositives) and stage IV (AIDS) of the CDC classification, by using an enzyme amplified sensitivity immunoassay. We measured the level of GM‐CSF and TNF α in supernatant of phytohemagglutinin‐activated peripheral blood mononuclear cells from patients and healthy individuals. In one out of 10 stage II patients and 4 out of 14 stage IV patients, we obtained higher levels of GM‐CSF than the mean + 2 S.D. of controls, but in 3 stage IV patients with very low CD4+ T lymphocyte counts (< 50/mm –3 ) compared to other patients, the GM‐CSF values were very low. High levels of TNF α were detected in 3 out of 10 stage II and 6 out of 11 stage IV patients. The high values of TNF α were associated with high values of GM‐CSF in stage II and in most of AIDS patients except those with very low CD4 + T cell counts, who produced low levels of GM‐CSF. Plasma levels of cytokines were evaluated in 10 stage II, 22 stage IV patients and 20 controls. Increased levels of GM‐CSF (more than 9 pg/ml) were observed in the plasma from 8 out of 10 stage II patients and 17 out of 22 stage IV patients. The tendency that increased levels of GM‐CSF were associated with increased levels of TNF α was observed in plasma from stage IV patients. We report a disarray of GM‐CSF production in patients with HIV infection that could be involved in clinical manifestations and progression of the disease.