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Production of Monoclonal Anti‐Idiotype Antibodies Which Mimic an M‐Like Protein of Streptococcus equi
Author(s) -
JeanFrancois M. J. Bernadette,
Poskitt David C.,
MacDonald Lisa M.,
Turnbull Sally J.,
Yasmeen Dilruba
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01699.x
Subject(s) - monoclonal antibody , idiotype , antibody , biology , paratope , antigen , microbiology and biotechnology , chemistry , immunology
Rat monoclonal anti‐idiotype antibodies (mAb2) were raised against two mouse monoclonal antibodies (mAb1), 1D10 and 2A6, with specificity for the M‐like protein of Streptococcus equi. The capacity of the mAb2 to inhibit the binding between the corresponding mouse mAbl against which the mAb2 were raised and the M‐like protein was investigated in an inhibition EIA. One of the ten mAb2 examined, namely 5D1 (anti‐mAb1 1D10), was able to inhibit this binding. The mAb2 5D1 bound to the mAb1 1D10 in such a way as to completely inhibit the subsequent binding of the M‐like protein antigen to the paratope of the mAb1 1D10. The mAb2 5D1 is likely to represent a true image of the M‐like protein antigen and may thus be described as an Ab2β anti‐idiotype antibody.