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Immunogenicity of Vibrio cholerae O1 Fimbriae in Animal and Human Cholera
Author(s) -
Ehara Masahiko,
Ichinose Yoshio,
Iwami Mamoru,
Utsunomiya Akiyoshi,
Shimodori Shoichi,
Kangethe Stanley K.,
Neves Bianca C.,
Supawat Krongkaew,
Nakamura Satoshi
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01692.x
Subject(s) - vibrio cholerae , fimbria , immunogenicity , microbiology and biotechnology , cholera , cholera vaccine , biology , vibrionaceae , serotype , polyclonal antibodies , bacterial adhesin , immunization , virology , antibody , antigen , immunology , bacteria , escherichia coli , biochemistry , genetics , gene
Parenteral immunization with either formalin‐fixed whole cells of the fimbriate Bgd17 strain or purified fimbriae protected against Vibrio cholerae O1 infection in rabbits, independent of biotype and serotype. Parenteral immunization of adult rabbits with purified fimbriae prior to V. cholerae O1 challenge resulted in a reduction of 2 to 3 orders of magnitude in the number of bacteria recovered from the small intestines of immunized rabbits in comparison to non‐immunized controls. IgG and IgA antibodies against fimbrillin of V. cholerae O1 were detected in the convalescent sera of patients with cholera; however, little fimbrial antigen was detected in the commercially available cholera vaccines when examined by polyclonal and monoclonal antibodies against fimbriae. These data suggest that fimbrial hemagglutinin is a major adhesin of V. cholerae O1 and that parenteral immunization with fimbriae generates a specific immune response in the gut that may serve as one means of mitigating subsequent V. cholerae O1 gut infection.