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Release of Prostaglandin E 2 by Human Mononuclear Cells Exposed to Heat‐Killed Salmonella typhi
Author(s) -
Yusof Wan Noraini Wan,
Nagaratnam Menaka,
Koh Chonglek,
Puthucheary Savithri,
Pang Tikki
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01690.x
Subject(s) - salmonella typhi , leukotriene b4 , metabolite , peripheral blood mononuclear cell , biology , prostaglandin e2 , leukotriene c4 , in vitro , leukotriene , immunoassay , salmonella , prostaglandin , arachidonic acid , microbiology and biotechnology , prostaglandin e , enzyme , biochemistry , bacteria , immunology , escherichia coli , endocrinology , antibody , genetics , asthma , inflammation , gene
Human mononuclear cells pre‐labeled with [ 3 H]arachidonic acid were shown to release metabolites following in vitro addition of heat‐killed Salmonella typhi (HKST). The amount of label released was significantly higher than that seen with live S. typhi (LST). Addition of increasing amounts of HKST resulted in an increased release of metabolites. Enzyme immunoassay of the culture supernatants revealed that the bulk of the metabolite released was prostaglandin E 2 (PGE 2 ). Leukotriene B 4 (LTB 4 ) and leukotriene C 4 (LTC 4 ) were not detectable in the culture supernatants. The significance and implications of these results are discussed.