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Host‐Specific Hemagglutination of Influenza A (H1N1) Virus
Author(s) -
Morishita Takayuki,
Kobayashi Shinichi,
Miyake Takashi,
Ishihara Yuichi,
Nakajima Setsuko,
Nakajima Katsuhisa
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01689.x
Subject(s) - biology , hemagglutination , hemagglutinin (influenza) , hemagglutination assay , virus , virology , antiserum , host (biology) , amino acid , microbiology and biotechnology , antigen , genetics , titer
Abstract H1N1 strains of influenza A virus isolated during the influenza season of 1991–92 were divided into two groups according to the property of host‐specific hemagglutination. Group 1 viruses agglutinated human and chicken red blood cells. Group 2 viruses agglutinated human but not chicken red blood cells. The viruses of both groups, however, showed the same antigenic structure determined with ferret antisera. The virus clones which were plaque‐purified twice from a group 2 virus retained the characteristic of host‐specific hemagglutination after five successive passages in MDCK cells, indicating that this phenomenon is genetically determined. However, the amino acid, sequences of the hemagglutinin (HA) polypeptides deduced from the nucleotide sequences of the HA gene of the two groups did not show any differences between them. This suggests a difference in amino acids in some other polypeptide(s), which affects the host‐specific hemagglutination.