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A Comparative Study on T Cell Receptor Vβ Gene Usages: Spleen Cells from the Non‐Obese Diabetic (NOD) Mouse and Its Non‐Diabetic Sister Strain, the ILI Mouse, and Infiltrating T Cells into Pancreata of NOD Mice
Author(s) -
Koide Yukio,
Kaidoh Toshiö,
Yanagawa Tatsuo,
Yoshida Takato O.
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01688.x
Subject(s) - insulitis , nod mice , nod , biology , t cell receptor , spleen , gene , microbiology and biotechnology , receptor , polymerase chain reaction , beta cell , immunology , t cell , endocrinology , diabetes mellitus , islet , immune system , genetics
We analyzed the usage of T cell receptor (TCR) Vβ genes of spleen cells of NOD mice in comparison with those of its non‐diabetic sister strain ILI mice which show no insulitis and (ILI × NOD)F 1 mice. The quantitative polymerase chain reaction (PCR) method revealed that PCR Vβ repertoires of these mice are indistinguishable. This is consistent with our previous observation that ILI mice share the same H‐2 class II genes with NOD mice. PCR method also revealed that the Vβ transcript of infiltrating T cells into pancreas of NOD mice was not restricted but was rather diverse. The role of TCR repertoire in the development of insulitis was discussed.