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Functional and Phenotypical Analysis of Subsets of Rat CD4 + T Cells
Author(s) -
Nagai Yumiko,
Inobe Manabu,
Kikuchi Kokichi,
Uede Toshimitsu
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01685.x
Subject(s) - biology , epitope , microbiology and biotechnology , monoclonal antibody , t cell , phenotype , cd3 , cd44 , antigen , gene expression , cell , gene , immunology , cd8 , antibody , genetics , immune system
Rat CD4 + T cells were divided into two distinct subsets by a monoclonal antibody RTH‐1 recognizing a unique epitope on rat CD45R. Cellular distribution of OX‐22‐ and RTH‐1‐defined antigens was the same. However, OX‐22 and RTH‐1 recognized different epitopes that exist on rat CD45R. The expression of IL‐4 gene was detected only in RTH‐1 low CD4+ T cell subset upon various stimulations. In contrast, the expression of IL‐2 and IFN‐γ gene varied depending upon the nature of stimuli. The increased cell surface expression of CD44 was detected in RTH‐1 high CD4+ T cell subset. Conversely the increased expression of CD2 was detected in RTH‐1 low CD4+ T cell subset. The expression of CD3 and LFA‐1 was not significantly different between RTH‐1 high and RTH‐1 low subsets.