Premium
Detection of Various Variant Verotoxin Genes in Escherichia coli by Polymerase Chain Reaction
Author(s) -
Lin Zaw,
Kurazono Hisao,
Yamasaki Shinji,
Takeda Yoshifumi
Publication year - 1993
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1993.tb01675.x
Subject(s) - amplicon , biology , polymerase chain reaction , vtec , escherichia coli , verocytotoxin , gene , bglii , genetics , restriction enzyme , inverse polymerase chain reaction , microbiology and biotechnology , multiplex polymerase chain reaction , ecori
We constructed common primers for the polymerase chain reaction to detect the genes for various Verotoxins reported, that is, VT1 (or SLT‐I), VT2 (or SLT‐II), VT2vha, VT2vhb, SLT‐IIv (or VT2vp1, VTe) and SLT‐IIva (or VT2vp2). A total of 80 Verocytotoxin‐producing Escherichia coli strains isolated from humans, domestic animals and meats gave a positive result by PCR with the designed common primers. Digestion by restriction endonucleases Bgl II and Eco T14I of the amplicon of the VT2vp2 gene gave specific bands of the expected sizes, but not of the amplicons of other VT genes, suggesting a possible method for identification of the VT2vp2 gene. Application of the PCR with the designed primers in diagnostic and epidemiological studies on VTEC infection is also discussed.