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Enteric Adenovirus Type 41 Isolates: Cloning, Physical Maps and Diversity in Restriction Enzyme Cleavage Pattern
Author(s) -
Yamashita Yumi,
Hotsubo Tomoyuki,
Nakata Shuji,
Yamaguchi Taihei,
Sanekata Takeshi,
Chiba Shuzo,
Homma Morio,
Fujinaga Kei
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02131.x
Subject(s) - restriction enzyme , biology , restriction fragment , restriction map , strain (injury) , virology , genome , cloning (programming) , hindiii , microbiology and biotechnology , cleavage (geology) , restriction site , genetics , molecular cloning , dna , gene , plasmid , peptide sequence , anatomy , paleontology , fracture (geology) , computer science , programming language
Adenovirus (Ad) type 40 and 41 DNAs were directly extracted from stool specimens of children with gastroenteritis. Two new strains of Ad41, Sanekata and Ehime strain, were cloned and their restriction maps were constructed. The left terminal end of the cloned Ad41 genome, Eco RI‐E fragment of the Sanekata strain and Eco RI‐F fragment of the Ehime strain, had transforming ability in rat 3Y1 cells. Only one of the 35 isolates of Ad40 tested showed a different restriction profile, while three different restriction profiles were found in DNAs from Ad41 isolates.