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Serological Cross‐Reaction between Intact and Chemically Modified Lipopolysaccharides of O1 Vibrio cholerae Inaba and Non‐O1 V. cholerae Bio‐Serogroup Hakata
Author(s) -
Isshiki Yasunori,
Haishima Yuji,
Kondo Seiichi,
Hisatsune Kazuhito
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02123.x
Subject(s) - vibrio cholerae , serology , heterologous , antiserum , microbiology and biotechnology , biology , polysaccharide , vibrionaceae , hemolysis , cross reactivity , antigen , bacteria , antibody , biochemistry , cross reactions , gene , immunology , genetics
Serological cross‐reaction of intact as well as chemically modified LPS from O1 Vibrio cholerae 569B (Inaba) with non‐O1 V. cholerae Hakata LPS, which contain α(1→2)‐linked N ‐acetyl perosamine‐homopolymer constituting their O polysaccharide chain, was studied by passive hemolysis test by using their LPS as antigen for sensitizing sheep red blood cells (SRBC). The N ‐deacylation of the α(1→2)‐linked linear 3‐deoxy‐tetronyl perosamine‐homopolymer constituting the O polysaccharide chain in 569B LPS resulted in virtual elimination of their serological reactivity with both homologous Inaba and heterologous Hakata antisera. Furthermore, when the resultant NH 2 groups of the N ‐deacylated perosamine‐homopolymers in 569B LPS were N ‐acylated with acetyl, propionyl or butanoyl groups, they markedly recovered the serological reactivity to a marked extent, in particular, their pronounced cross‐serological reactivity with heterologous Hakata antiserum. These results are believed to be compatible with the interpretation that the Inaba antigen factor C possessed by the two bacteria studied is related to the common occurrence of the N ‐acyl groups, regardless of what the acyl groups are, residing in the perosamine residues of the perosamine‐homopolymers constituting the O polysaccharide chain of their LPS.