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Killing of Escherichia coli by Mononuclear Phagocytes and Neutrophils Stimulated In Vitro with β‐1,3‐d‐Polyglucose Derivatives
Author(s) -
Busund LillTove Rasmussen,
Pål Øian Zbigniew Konopski,
Seljelid Rolf
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02120.x
Subject(s) - peripheral blood mononuclear cell , microbiology and biotechnology , biology , phagocytosis , in vitro , tumor necrosis factor alpha , peritoneal cavity , escherichia coli , interleukin 8 , macrophage , phagocyte , cytokine , immunology , biochemistry , gene , anatomy
Human monocytes, human peritoneal macrophages, mouse peritoneal macrophages and human peripheral neutrophils pretreated with β‐1,3‐d‐polyglucose derivatives showed pronounced bactericidal capacity to Escherichia coli compared to control cells. The increased bactericidal capacity was detectable in mononuclear phagocytes over a wide range of concentrations of bacteria. Granulocytes, however, showed bactericidal capacity only at low concentrations of bacteria. The pretreated mononuclear phagocytes released significant amounts of IL‐1 and PGE 2 . However, there was no significant release of tumor necrosis factor (TNF). By incubating unstimulated cells with purified IL‐1 and TNF, the bactericidal activity of neutrophils and mononuclear phagocytes was enhanced. Our data indicate that the inability of neutrophils stimulated with β‐1,3‐d‐polyglucose derivatives to kill large numbers of bacteria could be overcome by a combined treatment with purified IL‐1 or TNF in addition to β‐1,3‐D‐polyglucose derivatives. By incubating unstimulated cells with medium from β‐1,3‐d‐polyglucose‐treated human peritoneal macrophages, the bactericidal activity of the cells was enhanced to the same extent as cells pretreated with purified TNF and IL‐1. Cells incubated with IL‐1‐depleted medium from β‐1,3‐d‐polyglucose‐treated human peritoneal macrophages, showed reduced bactericidal activity compared to cells incubated with undepleted medium. These studies demonstrate that β‐1,3‐d‐polyglucose‐treated mononuclear phagocytes and neutrophils show enhanced bactericidal activity. The enhanced activity is partly caused by stimulation of the cells with IL‐1 released from mononuclear phagocytes and partly by other unknown effects of β‐1,3‐d‐polyglucose derivatives on both mononuclear phagocytes and neutrophils.