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Cloning and Nucleotide Sequence Analysis of a Chloramphenicol Acetyltransferase Gene from Vibrio anguillarum
Author(s) -
Zhao Jiang,
Aoki Takashi
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02072.x
Subject(s) - biology , vibrio anguillarum , chloramphenicol acetyltransferase , cloning (programming) , gene , nucleic acid sequence , sequence analysis , vibrio , genetics , microbiology and biotechnology , bacteria , reporter gene , gene expression , computer science , programming language
The chloramphenicol resistant gene ( cat ) encoding chloramphenicol acetyltransferase (CAT) in a transferable R plasmid (pJA7324) isolated from the fish pathogen Vibrio anguillarum strain PT24 was cloned into the plasmid vector pUC19. The nucleotide sequence analysis of 1,348 base pair DNA identified an open reading frame encoding a protein of 216 amino acid residues with a calculated molecular mass of 25,471 daltons. The predicted amino acid sequences for this cat gene are 37–69% homologous with other CAT proteins of both Gram‐negative and ‐positive bacteria. Colony hybridization performed with a Pvu II‐ Bam HI fragment including this cat gene as a probe, revealed that the same or similar chloramphenicol resistance genes existed among V. anguillarum isolates.