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Nucleotide Sequence of the Arginine Deiminase Gene of Mycoplasma hominis
Author(s) -
Harasawa Ryô,
Koshimizu Kaoru,
Kitagawa Masanari,
Asada Kiyozo,
Kato Ikunoshin
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02068.x
Subject(s) - mycoplasma hominis , arginine deiminase , biology , nucleic acid sequence , gene , arginine , mycoplasma , cytosine , genetics , polymerase chain reaction , microbiology and biotechnology , amino acid
The arginine deiminase gene of Mycoplasma hominis was amplified by the polymerase chain reaction, and its entire nucleotide sequence was determined. This gene consists of 1227 base pairs encoding 409 amino acids, and has 35.2% guanine plus cytosine content. Nucleotide sequence homologies of the arginine deiminase gene between M. hominis and M. arginini , and between M. hominis and M. orale were 82.1 and 80.8%, respectively, suggesting that this gene is highly conserved among arginine‐utilizing Mycoplasma species.