Premium
Heat‐Labile Toxin from Bordetella parapertussis Induces Contraction of Smooth Muscle Cells in Culture
Author(s) -
Nagai Masaaki,
Endoh Masahiko,
Burns Drusilla L.,
Nakase Yasukiyo
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02063.x
Subject(s) - contraction (grammar) , biology , chinese hamster ovary cell , toxin , muscle contraction , actin , myocyte , cell culture , hamster , medicine , endocrinology , microbiology and biotechnology , andrology , genetics
The ability of Bordetella heat‐labile toxin (HLT) to contract various types of cells in culture was examined. HLT from B. parapertussis induced contraction of cultured smooth muscle cells from trachea, intestine, uterus and vas deferens as well as from aorta. The time required for contraction decreased as the dose of B. parapertussis HLT increased from 3 to 100 MID/ml. Upon exposure of cells to concentrations of toxin greater than 100 MID/ml, at least 2 hr was required for contraction. HLT from B. parapertussis did not affect cultured cardiac or skeletal muscle cells within 8 hr after the exposure to HLT (100 MID/ml). No effect on other types of primary culture cells or established cells such as Chinese hamster ovary (CHO) cells has been described. These data indicate that the primary target cells for HLT might be smooth muscle cells.