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Type 1 Pili Enhance the Invasion of Salmonella braenderup and Salmonella typhimurium to HeLa Cells
Author(s) -
Horiuchi Sankichi,
Inagaki Yoshio,
Okamura Noboru,
Nakaya Rintaro,
Yamamoto Naoki
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02059.x
Subject(s) - pilus , hela , biology , microbiology and biotechnology , salmonella , mannose , adhesion , fimbria , infectivity , enterobacteriaceae , mutant , in vitro , bacteria , virology , escherichia coli , biochemistry , chemistry , genetics , gene , virus , organic chemistry
The relationship between type 1 pili‐associated adhesion and invasion to HeLa cells by Salmonella braenderup and S. typhimurium was studied. When the clinical isolates of these strains were grown in L‐broth, they showed both type 1 pili formation and mannose‐sensitive adhesion to HeLa cells. On the other hand, the type 1 pili‐defective mutants, which were obtained either by repeated subcultures on L‐agar plates or by the transposon Tn 1 ‐insertion mutagenesis of the S. braenderup and S. typhimurium strains, concomitantly lost mannose‐sensitive adhesion to HeLa cells. When the HeLa cells were incubated with Salmonella , the type 1 piliated strains invaded the HeLa cells with much higher infection rate than did the type 1 pili‐defective strains. The invasion of type 1 piliated strains to HeLa cells was markedly inhibited in the presence of d‐mannose. The infectivity of the strain, which lost type 1 pili but still had mannose‐resistant adhesion, was slightly higher than that of the strains defective in both mannose‐sensitive and mannose‐resistant adhesion. These results suggested that type 1 pili have a role in enhancing the invasion of S. braenderup and S. typhimurium to HeLa cells.