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Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies
Author(s) -
Sinha Raghu,
Arora Sunil K.,
Datta Usha,
Sehgal Shobha
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb02038.x
Subject(s) - monoclonal antibody , antigen , polyclonal antibodies , antiserum , biology , virology , leishmania donovani , antibody , leishmania , epitope , monoclonal , western blot , visceral leishmaniasis , microbiology and biotechnology , leishmaniasis , immunology , parasite hosting , biochemistry , world wide web , computer science , gene
Twenty‐one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani , Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti‐leishmania antisera for detection of circulating leishmania antigens in kala azar patients' sera in a competitive‐enzyme‐linked immunosorbent assay system (ELISA). The anti‐leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was however low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.