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Molecular Analysis of Virus‐Producing and Non‐Producing Clones Derived from a Defective SSPE Virus Yamagata‐1 Strain
Author(s) -
Haga Takeshi,
Komase Katsuhiro,
Yoshikawa Yasuhiro,
Yamanouchi Kazuya
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb01663.x
Subject(s) - biology , clone (java method) , virus , virology , strain (injury) , microbiology and biotechnology , complementary dna , messenger rna , cell culture , gene , genetics , anatomy
Two virus clones were isolated from a defective SSPE virus, the Yamagata‐1 strain, and designated as the YA and YF clones. The YA clone‐infected cells produced neither cell‐free virus nor cell‐associated virus, whereas the YF clone‐infected cells produced both cell‐associated and cell‐free virus. No difference of epitopes on structural proteins was observed between these two clones. Both clones had hemadsorption activity. Quantitation of structural protein by Western dot blots showed relatively a lower amount of M protein in the YA‐infected cells than that in the YF‐infected cells. The ratio, P plus M dicistronic/M monocistronic mRNA, in the YA‐infected cells was about twice that in the YF‐infected cells. Sequence analysis of cDNA corresponding to P plus M dicistronic mRNA revealed that the deduced M protein of the YF virus was smaller than that of the YA virus by five amino acids from the carboxy terminal. These results suggest that abundant production of P plus M dicistronic mRNA is responsible for the reduced amount of M protein in the non‐productive YA clone.