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Studies on Osmotic Stability of Liposomes Prepared with Bacterial Membrane Lipids by Carboxyfluorescein Release
Author(s) -
Nagamachi Eiko,
Hirai Yoshikazu,
Tomochika Kenichi,
Kanemasa Yasuhiro
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb01661.x
Subject(s) - liposome , osmotic pressure , cardiolipin , osmotic shock , membrane , chromatography , biology , biochemistry , chemistry , phospholipid , gene
The authors measured the osmotic stability of liposomes prepared with membrane lipids of bacteria, using the osmotic‐shock release of entrapped carboxyfluorescein as an indicator. The sub‐second physical changes of liposomes suspended in a solution of low osmotic pressure were examined by stopped flow spectrophotometry. The entrapped carboxyfluorescein was released when the liposomes burst on inflow of excess water. Liposomes prepared with the lipids of a stable Staphylococcus aureus L‐form strain were more resistant to low osmotic pressure than those prepared from the wild strain of S. aureus , and liposomes prepared from Mycoplasma orale were even more resistant. Cardiolipin enhanced the lipid membrane stability in S. aureus and cholesterol in M. orale. The stability of lipid membranes to low osmotic pressure could be precisely determined by the present method.