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Immunoelectron Microscopy of Chlamydia psittaci with Monoclonal Antibodies
Author(s) -
Ando Shuji,
Takashima Ikuo,
Hashimoto Nobuo
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb01648.x
Subject(s) - immunoelectron microscopy , monoclonal antibody , biology , chlamydia psittaci , virology , chlamydia , chlamydiaceae , microbiology and biotechnology , chlamydiales , microscopy , chlamydia trachomatis , antibody , immunology , pathology , medicine
An immunoelectron microscopic study was performed to determine the distribution of antigenic components on particles of Chlamydia psittaci and infected cells using a number of monoclonal antibodies (MAbs). Of three anti‐lipopolysaccharide (LPS) antibodies (4D5, A2 and 4G5), two antibodies (4D5 and A2) reacted with the surface of reticulate bodies (RBs) but not with that of elementary bodies (EBs). The other antibody (4G5) reacted with both EBs and RBs. Examination of infected cells in thin sections revealed that 4D5 and A2 combined with the membranes of both EBs and RBs. These results indicate that each LPS epitope localized at a different position in the chlamydial membrane. Most MAbs directed to protein antigens reacted on the surface of both EBs and RBs though 3E9 specific for the 90 kDa and 50 kDa protein components combined with RBs only.