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A Broad‐Host‐Range Vibriophage, KVP40, Isolated from Sea Water
Author(s) -
Matsuzaki Shigenobu,
Tanaka Shuji,
Koga Tetsuro,
Kawata Tomio
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb01645.x
Subject(s) - biology , ecori , host (biology) , vibrio , hindiii , restriction enzyme , vibrio parahaemolyticus , bamhi , dna , vibrionaceae , microbiology and biotechnology , genetics , bacteria
A broad‐host‐range vibriophage, KVP40, was isolated from sea water by using Vibrio parahaemolyticus 1010 (EB101) as the indicator host. The host range of KVP40 extended over at least 8 Vibrio and 1 Photobacterium species. KVP40 was a large tailed phage containing double‐stranded DNA and belonged to Ackermann's morphotype A2. KVP40 DNA was cleaved by 11 different type II restriction endo‐nucleases including EcoRl and Hindlll , but not by 17 other enzymes including Bam HI, Kpn I and Sal I.1