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Detection and Discrimination of Mycoplasma pneumoniae and Mycoplasma genitalium by the in vitro DNA Amplification
Author(s) -
Sasaki Yuko,
Shintani Miharu,
Shimada Toru,
Watanabe Haruo,
Sasaki Tsuguo
Publication year - 1992
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1992.tb01638.x
Subject(s) - mycoplasma genitalium , biology , mycoplasma pneumoniae , mycoplasmataceae , mycoplasma , mollicutes , in vitro , microbiology and biotechnology , virology , genetics , chlamydia trachomatis , pneumonia , medicine
By using the primers designed on the bases of the sequences of the 16S rRNA genes of Mycoplasma pneumoniae and Mycoplasma genitalium , respectively, specific and sensitive in vitro DNA amplification assay system for the detection and discrimination of these two mycoplasmas was established. The detection limit of the assay was 100 cells for M. pneumoniae and 1,000 cells for M. genitalium. Neither other human mycoplasmas nor oral bacteria existing in human saliva showed any cross‐reactions with these primers.