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Monoclonal Antibody to a Structure Expressed on a Subpopulation of Rat CD8 T Cell Subsets
Author(s) -
Nagoya Satoshi,
Kikuchi Kokichi,
Uede Toshimitsu
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb02029.x
Subject(s) - biology , cytotoxic t cell , microbiology and biotechnology , antibody , cd8 , monoclonal antibody , epitope , population , antigen , t cell , antigen presenting cell , immunology , immune system , biochemistry , in vitro , demography , sociology
We have developed a monoclonal antibody, RTS‐1, which can divide a rat CD8 (+) peripheral T cell population into two functionally distinct subsets. The cell‐surface structure defined by this antibody is a glycoprotein with a molecular weight of 220 kDa found to be a high molecular isoform of rat CD45 antigen. CD4 (+) T cells were not stained by RTS‐1 antibody. The cytotoxic T cell‐enriched population did not express RTS‐1 epitope on the cell surface. CD8 (+) spleen cells as well as RTS‐1(+)CD8(+)T cells exhibited strong inhibition on mitogen‐induced immunoglobulin G production by rat B cells. Furthermore, RTS‐1 antibody, but not the control antibody, abolished CD8(+)T cell‐mediated inhibition of immunoglobulin G production by rat B cells. These data suggest that RTS‐1 antibody recognizes a unique determinant of rat CD45 antigen that is expressed on a fraction of CD8(+) cells.