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Interleukin‐4 Downregulates Interleukin‐6 Production by Human Alveolar Macrophages at Protein and mRNA Levels
Author(s) -
Yanagawa Hiroaki,
Sone Saburo,
Sugihara Kohji,
Tanaka Keiji,
Ogura Takeshi
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb02028.x
Subject(s) - biology , messenger rna , interleukin , alveolar macrophage , immunology , interleukin 1β , interleukin 4 , interleukin 15 , cytokine , macrophage , microbiology and biotechnology , biochemistry , in vitro , gene
Effects of interleukin‐4 (IL‐4) on IL‐6 production by human alveolar macrophages (AM) obtained by bronchoalveolar lavage from healthy donors was examined at the protein and gene levels. IL‐6 production was quantitated by enzyme immunoassay (EIA) and bioassay using the IL‐6 dependent murine hybridoma cell line MH60.BSF2. Results showed that when activated with LPS, AM released significantly more biologically active IL‐6 than blood monocytes. Human rIL‐4 significantly suppressed IL‐6 production by AM and monocytes stimulated with LPS. Northern blot analysis revealed that IL‐4 reduced the expression of IL‐6 mRNA in LPS‐stimulated AM and monocytes. The inhibitory effect was most pronounced when IL‐4 was added with LPS or within the first 4 hr after LPS to AM or monocytes. The suppressive effect of IL‐4 was completely neutralized by pretreatment with anti‐IL‐4 antibody. IL‐4 also showed a suppressive effect on IL‐6 production by macrophages generated in vitro by maturation of blood monocytes with granulocyte‐macrophage colony stimulating factor (GM‐CSF). These observations suggest that IL‐4 may play a critical role in in situ regulation of immune responses through suppression of IL‐6 production.