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Immune Response of Mice Infected with Recombinant Vaccinia Viruses Carrying the HIV gag Gene
Author(s) -
Sugata Fumihiko,
Aoki Naoto,
Shioda Tatsuo,
Hayashi Takuma,
Shimada Kaoru,
Mitamura Keiji,
Shibuta Hiroshi
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb02025.x
Subject(s) - group specific antigen , vaccinia , biology , virology , immune system , recombinant dna , poxviridae , virus , orthopoxvirus , cellular immunity , antigen , hiv antigens , antibody , microbiology and biotechnology , gene , immunology , viral disease , biochemistry
We examined mouse immune response to 4 kinds of recombinant vaccinia viruses carrying the HIV gag gene, including vac‐gag/pol, which produces HIV‐like particles with processed gag proteins; vac‐gag, which also produces HIV‐like particles but with unprocessed gag protein; and vac‐gag‐pol‐fuse and vac‐es‐gag/pol, neither of which produces such particles but releases reverse transcriptase and gag protein, respectively, from infected cells. Although infection of mice with recombinant vaccinia viruses induced production of the anti‐p24 antibody in all mice, vac‐gag/pol and vac‐es‐pol induced higher production than the other two recombinants. Increase in [ 3 H]thymidine uptake by splenic lymphocytes following p24 antigen stimulation was most evident in mice infected with vac‐gag/pol. Thus, the highest immune reaction, both humoral and cellular, was elicited by vac‐gag/pol, indicating that among those tested, this recombinant vaccinia virus is the best candidate for a vaccine that induces anti‐HIV gag immunity.