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Two Generalized Transducing Phages in Vibrio parahaemolyticus and Vibrio alginolyticus
Author(s) -
Muramatsu Kouichi,
Matsumoto Hideki
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01629.x
Subject(s) - lysogenic cycle , vibrio parahaemolyticus , biology , vibrio alginolyticus , microbiology and biotechnology , vibrio , bacteriophage , transduction (biophysics) , prophage , lysis , strain (injury) , bacteria , vibrio infections , escherichia coli , gene , genetics , biochemistry , anatomy
Two bacteriophages named φVP253 and φVP143 isolated after ultraviolet induction from lysogenic strains of Vibrio parahaemolyticus have been shown to be generalized transducing phages. So far, seven different auxotrophic markers of a V. parahaemolyticus strain could be transduced at the frequencies ranging from 2.2× 10 −7 to 7.5×10 −5 per infected cell at the m.o.i. of approximately 1.0. The phage φVP143, but not φVP253, lysed 20 of the 28 strains of V. alginolyticus and the occurrence of generalized transduction by this phage in this Vibrio species has been confirmed. Molecular size of the genomes of both phages were estimated to be approximately 48 kb as judged from electrophoretic mobilities of the DNAs digested with Hin dIII endonuclease. The results and similarity of the two phages in morphology and other properties suggest very close relatedness of the phages.