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Antibacterial Activity of Cefpodoxime against Branhamella catarrhalis
Author(s) -
Takenouchi Takashi,
Nishino Takeshi
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01628.x
Subject(s) - cefpodoxime , biology , microbiology and biotechnology , antibacterial agent , lysis , bacteria , antibacterial activity , antibiotics , genetics
The antibacterial activity of cefpodoxime against Branhamella catarrhalis was studied. All of the 65 clinical isolates tested were inhibited at and below 1.56 μg/ml, both at 10 7 and at 10 5 CFUs. The following was further studied on B. catarrhalis N‐5 which showed average susceptibility to each drug examined. Bactericidal activity was observed at and above the MIC. Scanning and transmission electron microscopy revealed morphological changes, such as cellular swelling, bleb formation, inhibition of septum formation, and lysis, of the cells exposed to cefpodoxime at concentrations around the MIC. Cefpodoxime was poorly hydrolyzed by the β‐lactamase and it showed affinity for two penicillin‐binding proteins that had approximate molecular weights of 83 and 74 kilodaltons, with I 50 values of 3.7 and 2.1 μg/ml, respectively.